Biogenesis and quality control of the mitochondrial outer membrane

Doron Rapaport

DoronRapaport23-11-2012-09 crop1
  • PhD work 1992-1995 at the Weizmann Institute of Science, Rehovot
  • Postdoctoral training 1996-2001 at the University of Munich
  • Group leader 2002-2006 at the University of Munich
  • Professor of Biochemistry at the Interfaculty Institute of Biochemistry since 2006

Research Interest

The mitochondrial outer membrane contains a diverse set of proteins including enzymes, pore-forming proteins, regulators of programmed cell death, and those that control the morphology of the organelle.

We investigate the molecular mechanisms by which the various mitochondrial outer membrane proteins are targeted to mitochondria, inserted into the outer membrane and assembled into functional complexes. In addition we aim to understand how proteins that are mistargeted to mitochondria are recognized as such and subsequently removed. We further study the exchange of lipids between mitochondria and other cellular compartments. For our studies we use both yeast and mammalian tissue cultures as experimental systems and employ a variety of biochemical, molecular cell biology and genetics assays.

  • overview import 4
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Import pathways of mitochondrial proteins

Available PhD Projects

Project 1: Studying quality control of mitochondrial outer membrane proteins.

Project 2: Understanding the mechanisms by which phospholipids are exchanged between mitochondria and other organelles.

Selected Reading

1) Sinzel, M., T. Tan, P. Wendling, H. Kalbacher, C. Özbalci, X. Chelius, B. Westermann, B. Brügger, D. Rapaport and K.S. Dimmer (2016) Mcp3 is a novel mitochondrial outer membrane protein that follows a unique IMP-dependent biogenesis pathway. EMBO Rep. 17, 965-981.

2) Jores, T., A. Klinger, L. Groß, S. Kawano, N. Flinner, E. Duchardt-Ferner, J. Wöhnert, H. Kalbacher, T. Endo, E. Schleiff, and D. Rapaport (2016) Characterization of the targeting signal in mitochondrial β-barrel proteins. Nature Comm. 7, 12036.

3) Dimmer, K.S., and D. Rapaport (2017) Mitochondrial contact sites as platforms for phospholipid exchange. Biochim. Biophys. Acta 1862, 69-80.